Ubiquitous Lens α-, β-, and γ-Crystallins Accumulate in Anuran Cornea as Corneal Crystallins
نویسندگان
چکیده
منابع مشابه
Age-dependent association of γ-crystallins with aged α-crystallins from old bovine lens
PURPOSE Previous theoretical and experimental studies have predicted that the loss of weak protein interactions between alpha- and gamma-crystallins could result in a decrease in the transparent properties of the aging lens. METHODS alpha-Crystallins were prepared from the nucleus of old bovine lens, and gamma-crystallins were prepared from whole fetal bovine lens or from the nucleus of old b...
متن کاملPhysiological expression of lens α-, β-, and γ-crystallins in murine and human corneas
PURPOSE How corneal transparency is formed/maintained remains largely unclear. A group of enzymes which are referred to as enzymatic crystallins were proposed to contribute to corneal transparency in various animals. This study investigated whether the three classical lens crystallins, namely α-, β-, and γ-crystallins, exist in mouse and human corneas. METHODS Mice, human tissues, and culture...
متن کاملMolecular diversity and genomic organisation of the α, β and γ eye lens crystallins from the Antarctic toothfish Dissostichus mawsoni
The eye lens of the Antarctic toothfish living in the −2 °C Southern Ocean is cold-stable. To investigate the molecular basis of this cold stability, we isolated, cloned and sequenced 22 full length crystallin cDNAs. We found two α crystallins (αA, αB), six β crystallins (βA1, βA2, βA4, βB1, βB2, βB3) and 14 γ crystallins (γN, γS1, γS2, γM1, γM3, γM4, γM5, γM7, γM8a, γM8b, γM8c, γM8d, γM8e, and...
متن کاملα- and β-crystallins modulate the head group order of human lens membranes during aging.
PURPOSE To examine the physical properties of human lens cell membranes as a function of age. METHODS The environment of the phospholipid head groups in fiber cell membranes from human lenses, aged 22 to 83 years, was assessed with Laurdan and two-photon confocal microscopy. The effect of mild thermal stress on head group order was studied with lens pairs in which one intact lens was incubate...
متن کاملCleavage of β-Crystallins During Maturation of Bovine Lens
Results: The identities of the major crystallins and several additional crystallin species missing portions of their Nterminal extensions were identified in the fetal bovine lens. Besides the previously identified form of βB1 missing 15 residues from its N-terminus, forms of βA3 missing 11 and 22 residues were identified. With aging, the βA3 (-22) species became a major protein in the adult bov...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2007
ISSN: 0021-9258
DOI: 10.1074/jbc.m609275200